| | |  | | | | Record from Web of Science® | | | | | | |
| Genomic sequencing of Pleistocene cave bears |
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| Author(s): Noonan JP, Hofreiter M, Smith D, Priest JR, Rohland N, Rabeder G, Krause J, Detter JC, Paabo S, Rubin EM |
| Source: SCIENCE Volume: 309 Issue: 5734 Pages: 597-600 Published: JUL 22 2005 |
| Times Cited: 71 References: 18 |
| Abstract: Despite the greater information content of genomic DNA, ancient DNA studies have largely been limited to the amplification of mitochondrial sequences. Here we describe metagenomic libraries constructed with unamplified DNA extracted from skeletal remains of two 40,000-year-old extinct cave bears. Analysis of similar to 1 megabase of sequence from each library showed that despite significant microbial contamination, 5.8 and 1.1% of clones contained cave bear inserts, yielding 26,861 base pairs of cave bear genome sequence. Comparison of cave bear and modern bear sequences revealed the evolutionary relationship of these lineages. The metagenomic approach used here establishes the feasibility of ancient DNA genome sequencing programs. |
| Document Type: Article |
| Language: English |
| Reprint Address: Rubin, EM (reprint author), US Dept Energy Joint Genome Inst, Walnut Creek, CA 94598 USA |
Addresses:
1. US Dept Energy Joint Genome Inst, Walnut Creek, CA 94598 USA
2. Univ Calif Berkeley, Lawrence Berkeley Lab, Genom Div, Berkeley, CA 94720 USA
3. Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany
4. Univ Vienna, Inst Paleontol, A-1010 Vienna, Austria
5. Lawrence Livermore Natl Lab, Biosci Directorate, Livermore, CA 94550 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 948SE |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1113485 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |