| | |  | | | | Record from Web of Science® | | | | | | |
| Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells |
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| Author(s): Cowan CA, Atienza J, Melton DA, Eggan K |
| Source: SCIENCE Volume: 309 Issue: 5739 Pages: 1369-1373 Published: AUG 26 2005 |
| Times Cited: 251 References: 28 |
| Abstract: We have explored the use of embryonic stem cells as an alternative to oocytes for reprogramming human somatic nuclei. Human embryonic stem (hES) cells were fused with human fibroblasts, resulting in hybrid cells that maintain a stable tetraploid DNA content and have morphology, growth rate, and antigen expression patterns characteristic of hES cells. Differentiation of hybrid cells in vitro and in vivo yielded cell types from each embryonic germ layer. Analysis of genome-wide transcriptional activity, reporter gene activation, allele-specific gene expression, and DNA methylation showed that the somatic genome was reprogrammed to an embryonic state. These results establish that hES cells can reprogram the transcriptional state of somatic nuclei and provide a system for investigating the underlying mechanisms. |
| Document Type: Article |
| Language: English |
| Reprint Address: Eggan, K (reprint author), Harvard Univ, Howard Hughes Med Inst, Harvard Stem Cell Inst, Dept Mol & Cellular Biol, 7 Divin Ave, Cambridge, MA 02138 USA |
Addresses:
1. Harvard Univ, Howard Hughes Med Inst, Harvard Stem Cell Inst, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 959TX |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1116447 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |