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| Quantitative and dynamic assessment of the contribution of the ER to phagosome formation |
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| Author(s): Touret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S |
| Source: CELL Volume: 123 Issue: 1 Pages: 157-170 Published: OCT 7 2005 |
| Times Cited: 102 References: 64 |
| Abstract: Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To reconcile these seemingly disparate hypotheses, we used a combination of biochemical, fluorescence imaging, and electron microscopy techniques to quantitatively and dynamically assess the contribution of the plasmalemma and of the ER to phagosome formation and maturation. We could not verify even a transient physical continuity between the ER and the plasma membrane, nor were we able to detect a significant contribution of the ER to forming or maturing phagosomes in either macrophages or dendritic cells. Instead, our data indicate that the plasma membrane is the main constituent of nascent and newly formed phagosomes, which are progressively remodeled by fusion with endosomal and eventually lysosomal compartments as phagosomes mature into acidic, degradative organelles. |
| Document Type: Article |
| Language: English |
| Reprint Address: Grinstein, S (reprint author), Hosp Sick Children, Programme Cell Biol, 555 Univ Ave, Toronto, ON M5G 1X8 Canada |
Addresses:
1. Hosp Sick Children, Programme Cell Biol, Toronto, ON M5G 1X8 Canada
2. Hosp Sick Children, Gastroenterol Hepatol & Nutr Dept, Toronto, ON M5G 1X8 Canada
3. Univ Toronto, Dept Biochem, Toronto, ON M5G 1X8 Canada
4. Univ Toronto, Dept Life Sci, Toronto, ON M5G 1X8 Canada
5. Univ Calif Berkeley, Dept Cell & Mol Biol, Berkeley, CA 94720 USA
6. Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA
7. Netherlands Canc Inst, NL-1066 CX Amsterdam, Netherlands
8. Univ Mediterranee, CNRS, INSERM, Ctr Immunol Marseille Luminy, F-13288 Marseille, France |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 973QD |
| ISSN: 0092-8674 |
| DOI: 10.1016/j.cell.2005.08.018 |
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