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| Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts |
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| Author(s): Meissner A, Jaenisch R |
| Source: NATURE Volume: 439 Issue: 7073 Pages: 212-215 Published: JAN 12 2006 |
| Times Cited: 90 References: 18 |
| Abstract: The derivation of embryonic stem (ES) cells by nuclear transfer holds great promise for research and therapy but involves the destruction of cloned human blastocysts. Proof of principle experiments have shown that 'customized' ES cells derived by nuclear transfer (NT-ESCs) can be used to correct immunodeficiency in mice. Importantly, the feasibility of the approach has been demonstrated recently in humans, bringing the clinical application of NT- ESCs within reach. Altered nuclear transfer (ANT) has been proposed as a variation of nuclear transfer because it would create abnormal nuclear transfer blastocysts that are inherently unable to implant into the uterus but would be capable of generating customized ES cells. To assess the experimental validity of this concept we have used nuclear transfer to derive mouse blastocysts from donor fibroblasts that carried a short hairpin RNA construct targeting Cdx2. Cloned blastocysts were morphologically abnormal, lacked functional trophoblast and failed to implant into the uterus. However, they efficiently generated pluripotent embryonic stem cells when explanted into culture. |
| Document Type: Article |
| Language: English |
| Reprint Address: Jaenisch, R (reprint author), MIT, Whitehead Inst, 9 Cambridge Ctr, Cambridge, MA 02142 USA |
Addresses:
1. MIT, Whitehead Inst, Cambridge, MA 02142 USA
2. MIT, Dept Biol, Cambridge, MA 02142 USA |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 001MT |
| ISSN: 0028-0836 |
| DOI: 10.1038/nature04257 |
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