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| The human SWI/SNF subunit Brm is a regulator of alternative splicing |
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| Author(s): Batsche E, Yaniv M, Muchardt C |
| Source: NATURE STRUCTURAL & MOLECULAR BIOLOGY Volume: 13 Issue: 1 Pages: 22-29 Published: JAN 2006 |
| Times Cited: 94 References: 50 |
| Abstract: The SWI/SNF (mating-type switch/sucrose nonfermenting) complex involved in chromatin remodeling on promoters has also been detected on the coding region of genes. Here we show that SWI/SNF can function as a regulator of alternative splicing. We found that the catalytic subunit Brm favors inclusion of variant exons in the mRNA of several genes, including E-cadherin, BIM, cyclin D1 and CD44. Consistent with this, Brm associates with several components of the spliceosome and with Sam68, an ERK-activated enhancer of variant exon inclusion. Examination of the CD44 gene revealed that Brm induced accumulation of RNA polymerase II (RNAPII) with a modified CTD phosphorylation pattern on regions encoding variant exons. Altogether, our data suggest that on genes regulated by SWI/SNF, Brm contributes to the crosstalk between transcription and RNA processing by decreasing RNAPII elongation rate and facilitating recruitment of the splicing machinery to variant exons with suboptimal splice sites. |
| Document Type: Article |
| Language: English |
| Reprint Address: Muchardt, C (reprint author), Inst Pasteur, Dept Dev Biol, CNRS, FRE 2850, Paris, France |
Addresses:
1. Inst Pasteur, Dept Dev Biol, CNRS, FRE 2850, Paris, France |
| Publisher: NATURE PUBLISHING GROUP, 75 VARICK STREET, 9TH FLOOR, NEW YORK, NY 10013-1917 USA |
| Subject Category: Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| IDS Number: 001TY |
| ISSN: 1545-9985 |
| DOI: 10.1038/nsmb1030 |
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