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| Coherence and timing of cell cycle start examined at single-cell resolution |
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| Author(s): Bean JM, Siggia ED, Cross FR |
| Source: MOLECULAR CELL Volume: 21 Issue: 1 Pages: 3-14 Published: JAN 6 2006 |
| Times Cited: 35 References: 54 |
| Abstract: Cell cycle '' Start '' in budding yeast involves induction of a large battery of G1/S-regulated genes, coordinated with bud morphogenesis. It is unknown how intra-Start coherence of these events and inter-Start timing regularity are achieved. We developed quantitative time-lapse fluorescence microscopy on a multicell-cycle timescale, for following expression of unstable GFP under control of the G1 cyclin CLN2 promoter. Swi4, a major activator of the G1/S regulon, was required for a robustly coherent Start, as swi4 cells exhibited highly variable loss of cooccurrence of regular levels of CLN2pr-GFP expression with budding. In contrast, other known Start regulators Mbp1 and CIn3 are not needed for coherence but ensure regular timing of Start onset. The interval of nuclear retention of Whi5, a Swi4 repressor, largely accounts for wildtype mother-daughter asymmetry and for variable Start timing in cln3 mbp1 cells. Thus, multiple pathways may independently suppress qualitatively different kinds of noise at Start. |
| Document Type: Article |
| Language: English |
| Reprint Address: Cross, FR (reprint author), Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA |
Addresses:
1. Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 004PM |
| ISSN: 1097-2765 |
| DOI: 10.1016/j.molcel.2005.10.035 |
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