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| Metagenomics to paleogenomics: Large-scale sequencing of mammoth DNA |
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| Author(s): Poinar HN, Schwarz C, Qi J, Shapiro B, MacPhee RDE, Buigues B, Tikhonov A, Huson DH, Tomsho LP, Auch A, Rampp M, Miller W, Schuster SC |
| Source: SCIENCE Volume: 311 Issue: 5759 Pages: 392-394 Published: JAN 20 2006 |
| Times Cited: 130 References: 26 |
| Abstract: We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics. |
| Document Type: Article |
| Language: English |
| Reprint Address: Poinar, HN (reprint author), McMaster Univ, McMaster Ancient DNA Ctr, 1280 Main St W, Hamilton, ON L8S 4L9 Canada |
Addresses:
1. McMaster Univ, McMaster Ancient DNA Ctr, Hamilton, ON L8S 4L9 Canada
2. McMaster Univ, Dept Anthropol, Hamilton, ON L8S 4L9 Canada
3. McMaster Univ, Dept Pathol & Mol Med, Hamilton, ON L8S 4L9 Canada
4. Penn State Univ, Ctr Comparat Genom & Bioinformat, University Pk, PA 16802 USA
5. Univ Oxford, Dept Zool, Henry Wellcome Ancient Biomol Ctr, Oxford OX1 3PS, England
6. Amer Museum Nat Hist, Div Vertebrate Zool Mammal, New York, NY 10024 USA
7. Russian Acad Sci, Inst Zool, St Petersburg 199034, Russia
8. Univ Tubingen, Inst Comp Sci, Ctr Bioinformat, D-72076 Tubingen, Germany
9. Garching Comp Ctr, D-85748 Garching, Germany |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 007AE |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1123360 |
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