| | |  | | | | Record from Web of Science® | | | | | | |
| The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway |
|
|
| Author(s): Bhattacharyya RP, Remenyi A, Good MC, Bashor CJ, Falick AM, Lim WA |
| Source: SCIENCE Volume: 311 Issue: 5762 Pages: 822-826 Published: FEB 10 2006 |
| Times Cited: 69 References: 38 |
| Abstract: Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory rote, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties. |
| Document Type: Article |
| Language: English |
| Reprint Address: Lim, WA (reprint author), Univ Calif San Francisco, Dept Mol & Cellular Pharmacol, 600 16th St, San Francisco, CA 94143 USA |
Addresses:
1. Univ Calif San Francisco, Dept Mol & Cellular Pharmacol, San Francisco, CA 94143 USA
2. Univ Calif San Francisco, Program Biol Sci, San Francisco, CA 94143 USA
3. Univ Calif San Francisco, Grad Grp Biophys, San Francisco, CA 94143 USA
4. Univ Calif Berkeley, Howard Hughes Med Inst, Mass Spectrometry Lab, Berkeley, CA 94720 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 012XT |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1120941 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |