| | |  | | | | Record from Web of Science® | | | | | | |
| In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production |
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| Author(s): Bro C, Regenberg B, Forster J, Nielsen J |
| Source: METABOLIC ENGINEERING Volume: 8 Issue: 2 Pages: 102-111 Published: MAR 2006 |
| Times Cited: 36 References: 36 |
| Abstract: In silico genome-scale cell models are promising tools for accelerating the design of cells with improved and desired properties. We demonstrated this by using a genome-scale reconstructed metabolic network of Saccharomyces cerevisiae to score a number of strategies for metabolic engineering of the redox metabolism that will lead to decreased glycerol and increased ethanol yields on glucose under anaerobic conditions. The best-scored strategies were predicted to completely eliminate formation of glycerol and increase ethanol yield with 10%. We successfully pursued one of the best strategies by expressing a non-phosphorylating, NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase in S. cerevisiae. The resulting strain had a 40% lower glycerol yield on glucose while the ethanol yield increased with 3% without affecting the maximum specific growth rate. Similarly, expression of GAPN in a strain harbouring xylose reductase and xylitol dehydrogenase led to an improvement in ethanol yield by up to 25% on xylose/glucose mixtures. (c) 2005 Elsevier Inc. All rights reserved. |
| Document Type: Article |
| Language: English |
| Reprint Address: Nielsen, J (reprint author), Tech Univ Denmark, Ctr Microbial Biotechol, Bioctr, Bldg 223, DK-2800 Lyngby, Denmark |
Addresses:
1. Tech Univ Denmark, Ctr Microbial Biotechol, Bioctr, DK-2800 Lyngby, Denmark
2. Fluxome Sci AS, DK-2800 Lyngby, Denmark |
| Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA |
| Subject Category: Biotechnology & Applied Microbiology |
| IDS Number: 022JX |
| ISSN: 1096-7176 |
| DOI: 10.1016/j.ymben.2005.09.007 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |