| | |  | | | | Record from Web of Science® | | | | | | |
| Pluripotency of spermatogonial stem cells from adult mouse testis |
|
|
| Author(s): Guan K, Nayernia K, Maier LS, Wagner S, Dressel R, Lee JH, Nolte J, Wolf F, Li MY, Engel W, Hasenfuss G |
| Source: NATURE Volume: 440 Issue: 7088 Pages: 1199-1203 Published: APR 27 2006 |
| Times Cited: 246 References: 30 |
| Abstract: Embryonic germ cells as well as germline stem cells from neonatal mouse testis are pluripotent and have differentiation potential similar to embryonic stem cells(1,2), suggesting that the germline lineage may retain the ability to generate pluripotent cells. However, until now there has been no evidence for the pluripotency and plasticity of adult spermatogonial stem cells (SSCs), which are responsible for maintaining spermatogenesis throughout life in the male(3). Here we show the isolation of SSCs from adult mouse testis using genetic selection, with a success rate of 27%. These isolated SSCs respond to culture conditions and acquire embryonic stem cell properties. We name these cells multipotent adult germline stem cells (maGSCs). They are able to spontaneously differentiate into derivatives of the three embryonic germ layers in vitro and generate teratomas in immunodeficient mice. When injected into an early blastocyst, SSCs contribute to the development of various organs and show germline transmission. Thus, the capacity to form multipotent cells persists in adult mouse testis. Establishment of human maGSCs from testicular biopsies may allow individual cell-based therapy without the ethical and immunological problems associated with human embryonic stem cells. Furthermore, these cells may provide new opportunities to study genetic diseases in various cell lineages. |
| Document Type: Article |
| Language: English |
| Reprint Address: Hasenfuss, G (reprint author), Univ Gottingen, Ctr Heart, Dept Cardiol & Pneumol, Robert Koch Str 40, D-37075 Gottingen, Germany |
Addresses:
1. Univ Gottingen, Ctr Heart, Dept Cardiol & Pneumol, D-37075 Gottingen, Germany
2. Univ Gottingen, Inst Human Genet, D-37073 Gottingen, Germany
3. Univ Gottingen, Dept Cellular & Mol Immunol, D-37073 Gottingen, Germany |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 036OG |
| ISSN: 0028-0836 |
| DOI: 10.1038/nature04697 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |