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| Global survey of organ and organelle protein expression in mouse: Combined proteomic and transcriptomic profiling |
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| Author(s): Kislinger T, Cox B, Kannan A, Chung C, Hu PZ, Ignatchenko A, Scott MS, Gramolini AO, Morris Q, Hallett MT, Rossant J, Hughes TR, Frey B, Emili A |
| Source: CELL Volume: 125 Issue: 1 Pages: 173-186 Published: APR 7 2006 |
| Times Cited: 145 References: 50 |
| Abstract: Organs and organelles represent core biological systems in mammals, but the diversity in protein composition remains unclear. Here, we combine subcellular fractionation with exhaustive tandem mass spectrometry-based shotgun sequencing to examine the protein content of four major organellar compartments (cytosol, membranes [microsomes], mitochondria, and nuclei) in six organs (brain, heart, kidney, liver, lung, and placenta) of the laboratory mouse, Mus musculus. Using rigorous statistical filtering and machine-learning methods, the subcellular localization of 3274 of the 4768 proteins identified was determined with high confidence, including 1503 previously uncharacterized factors, while tissue selectivity was evaluated by comparison to previously reported mRNA expression patterns. This molecular compendium, fully accessible via a searchable web-browser interface, serves as a reliable reference of the expressed tissue and organelle proteomes of a leading model mammal. |
| Document Type: Article |
| Language: English |
| Reprint Address: Emili, A (reprint author), Univ Toronto, Donnelly Ctr Cellular & Biomol Res, 160 Coll St,Room 914, Toronto, ON M5S 3E1 Canada |
Addresses:
1. Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5G 1L6 Canada
2. Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8 Canada
3. Hosp Sick Children, Dept Dev Biol, Toronto, ON M5G 1L7 Canada
4. Univ Toronto, Dept Comp Sci, Toronto, ON M5S 2E4 Canada
5. McGill Univ, Ctr Bioinformat, Montreal, PQ H3A 2B4 Canada |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 039NY |
| ISSN: 0092-8674 |
| DOI: 10.1016/j.cell.2006.01.044 |
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