| | |  | | | | Record from Web of Science® | | | | | | |
| Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release |
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| Author(s): Kittel RJ, Wichmann C, Rasse TM, Fouquet W, Schmidt M, Schmid A, Wagh DA, Pawlu C, Kellner RR, Willig KI, Hell SW, Buchner E, Heckmann M, Sigrist SJ |
| Source: SCIENCE Volume: 312 Issue: 5776 Pages: 1051-1054 Published: MAY 19 2006 |
| Times Cited: 99 References: 37 |
| Abstract: The molecular organization of presynaptic active zones during calcium influx - triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED ( stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity. |
| Document Type: Article |
| Language: English |
| Reprint Address: Heckmann, M (reprint author), Inst Klin Neurobiol, Josef Schneider Str 11, D-97080 Wurzburg, Germany |
Addresses:
1. Inst Klin Neurobiol, D-97080 Wurzburg, Germany
2. European Neurosci Inst Gottingen, D-37077 Gottingen, Germany
3. Lehrstuhl Genet & Neurobiol, D-97074 Wurzburg, Germany
4. Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 043UX |
| ISSN: 0036-8075 |
| DOI: 10.1126/science.1126308 |
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