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| Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage |
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| Author(s): Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N |
| Source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 345 Issue: 3 Pages: 926-932 Published: JUL 7 2006 |
| Times Cited: 45 References: 19 |
| Abstract: Human ES (hES) cell lines are considered to be a valuable resource for medical research and for applications in cell therapy and drug discovery. For such utilization of hES cells to be realized, however, protocols involved in the use of hES cells, such as those for establishment, propagation, and cryopreservation, have still to be improved. Here, we report on an efficient method for the establishment of hES cell lines and its detailed characterization. Additionally, we developed a new bulk-passaging technique that preserves the karyotypic integrity of hES cell lines when maintained in culture for up to 2 years. Finally, we show that a simplified vitrification cryopreservation technique is vastly superior to standard slow-cooling methods with respect to cell viability. These results provide valuable information that will assist in achieving the goal of the large-scale hES cell culture required for the application of hES cells to disease therapy. (c) 2006 Elsevier Inc. All rights reserved. |
| Document Type: Article |
| Language: English |
| Reprint Address: Suemori, H (reprint author), Kyoto Univ, Inst Frontier Med Sci, Stem Cell Res Ctr, Lab Embryon Stem Cell Res,Sakyo Ku, 53 Kawahara Cho, Kyoto 6068507, Japan |
Addresses:
1. Kyoto Univ, Inst Frontier Med Sci, Stem Cell Res Ctr, Lab Embryon Stem Cell Res,Sakyo Ku, Kyoto 6068507, Japan
2. Kyoto Univ, Isnt Frontier Med Sci, Dept Dev & Differentiat, Sakyo Ku, Kyoto 6068507, Japan |
| Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA |
| Subject Category: Biochemistry & Molecular Biology; Biophysics |
| IDS Number: 054AA |
| ISSN: 0006-291X |
| DOI: 10.1016/j.bbrc.2006.04.135 |
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