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| Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) |
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| Author(s): Rust MJ (Rust, Michael J.), Bates M (Bates, Mark), Zhuang XW (Zhuang, Xiaowei) |
| Source: NATURE METHODS Volume: 3 Issue: 10 Pages: 793-795 Published: OCT 2006 |
| Times Cited: 261 References: 15 |
| Abstract: We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution. |
| Document Type: Article |
| Language: English |
| Reprint Address: Zhuang, XW (reprint author), Harvard Univ, Dept Phys, 12 Oxford St, Cambridge, MA 02138 USA |
Addresses:
1. Harvard Univ, Dept Phys, Cambridge, MA 02138 USA
2. Harvard Univ, Div Engn & Appl Sci, Cambridge, MA 02138 USA
3. Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
4. Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Biochemical Research Methods |
| IDS Number: 090HU |
| ISSN: 1548-7091 |
| DOI: 10.1038/nmeth929 |
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