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| Multipotent embryonic Isl1(+) progenitor cells lead to cardiac, smooth muscle, and endothelial cell diversification |
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| Author(s): Moretti A (Moretti, Alessandra), Caron L (Caron, Leslie), Nakano A (Nakano, Atsushi), Lam JT (Lam, Jason T.), Bernshausen A (Bernshausen, Alexandra), Chen YH (Chen, Yinhong), Qyang YB (Qyang, Yibing), Bu L (Bu, Lei), Sasaki M (Sasaki, Mika), Martin-Puig S (Martin-Puig, Silvia), Sun YF (Sun, Yunfu), Evans SM (Evans, Sylvia M.), Laugwitz KL (Laugwitz, Karl-Ludwig), Chien KR (Chien, Kenneth R.) |
| Source: CELL Volume: 127 Issue: 6 Pages: 1151-1165 Published: DEC 15 2006 |
| Times Cited: 152 References: 49 |
| Abstract: Cardiogenesis requires the generation of endothelial, cardiac, and smooth muscle cells, thought to arise from distinct embryonic precursors. We use genetic fate-mapping studies to document that isl1(+) precursors from the second heart field can generate each of these diverse cardiovascular cell types in vivo. Utilizing embryonic stem (ES) cells, we clonally amplified a cellular hierarchy of isl1(+) cardiovascular progenitors, which resemble the developmental precursors in the embryonic heart. The transcriptional signature of isl1(+)/Nkx2.5(+)/flk1(+) defines a multipotent cardiovascular progenitor, which can give rise to cells of all three lineages. These studies document a developmental paradigm for cardiogenesis, where muscle and endothelial lineage diversification arises from a single cell-level decision of a multipotent isl1(+) cardiovascular progenitor cell (MICP). The discovery of ES cell-derived MICPs suggests a strategy for cardiovascular tissue regeneration via their isolation, renewal, and directed differentiation into specific mature cardiac, pacemaker, smooth muscle, and endothelial cell types. |
| Document Type: Article |
| Language: English |
| Reprint Address: Chien, KR (reprint author), Massachusetts Gen Hosp, Cardiovasc Res Ctr, Charles River Pl CPZN 3208,185 Cambridge St, Boston, MA 02114 USA |
Addresses:
1. Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA
2. Tech Univ Munich, Klinikum Rechts Isar, Med Klin 1, D-81675 Munich, Germany
3. Univ Calif San Diego, Inst Mol Med, La Jolla, CA 92093 USA
4. Univ Calif San Diego, Sch Med, Skaggs Sch Pharm, La Jolla, CA 92093 USA
5. Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
6. Harvard Stem Cell Inst, Cambridge, MA 02138 USA |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 119CY |
| ISSN: 0092-8674 |
| DOI: 10.1016/j.cell.2006.10.029 |
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