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| A new protocol for quantifying CD34(+) cells |
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| Author(s): Kikuchi-Taura A (Kikuchi-Taura, Akie), Soma T (Soma, Toshihiro), Matsuyama T (Matsuyama, Tomohiro), Stern DM (Stern, David M.), Taguchi A (Taguchi, Akihiko) |
| Source: TEXAS HEART INSTITUTE JOURNAL Volume: 33 Issue: 4 Pages: 427-429 Published: 2006 |
| Times Cited: 7 References: 8 |
| Abstract: Increasing evidence points to a role for circulating CD34-positive (CD34+) cells in vascular maintenance and neovascularization. Although there are established methods for evaluating absolute numbers of CD34+ cells in bone marrow or mobilized peripheral blood, there is no convenient and highly reproducible method for quantifying low numbers of CD34+ cells in blood samples, such as those from the peripheral blood of patients with cardiovascular disease. With current commonly used methods, the mean percentage of CD34+ cells in leukocyte fractions from such patients was only 0.02%, and the cumulative intra-assay coefficient of variation was similar to 30%. With use of the protocol described herein, actual counts of CD34+ cells increased similar to 5-fold and cumulative intra-assay coefficients of variation were reduced to similar to 7%. The new method is useful to precisely measure low numbers of CD34+ cells in samples, and it has potential as a screening tool to evaluate cardiovascular risk in large patient populations. |
| Document Type: Article |
| Language: English |
| Reprint Address: Taguchi, A (reprint author), Natl Cardiovasc Ctr, Dept Cerebrovasc Dis, 5-7-1 Fujishiro Dai, Suita, Osaka 5658565 Japan |
Addresses:
1. Natl Cardiovasc Ctr, Dept Cerebrovasc Dis, Suita, Osaka 5658565 Japan
2. Univ Cincinnati, Coll Med, Cincinnati, OH 45221 USA
3. Natl Hosp Org, Osaka Minami Med Ctr, Dept Clin Lab, Osaka 5868521, Japan |
| Publisher: TEXAS HEART INST, PO BOX 20345, HOUSTON, TX 77225-0345 USA |
| Subject Category: Cardiac & Cardiovascular Systems |
| IDS Number: 120PS |
| ISSN: 0730-2347 |
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