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| IDENTIFICATION OF A 2ND HUMAN NM23 GENE, NM23-H2 |
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| Author(s): STAHL JA, LEONE A, ROSENGARD AM, PORTER L, KING CR, STEEG PS |
| Source: CANCER RESEARCH Volume: 51 Issue: 1 Pages: 445-449 Published: JAN 1 1991 |
| Times Cited: 395 References: 17 |
| Abstract: Reduced RNA and/or protein levels corresponding to the murine nm23-1 and human nm23-H1 complementary DNA clones have been correlated with high tumor metastatic potential in several rodent model systems and human breast carcinomas. We report the identification of a second human nm23 gene, designated nm23-H2. The pNM23-H2S complementary DNA clone predicted a M(r) 17,000 protein 88% identical to nm23-H1. nm23-H2 also shared a significant homology with nucleoside diphosphate kinases and a Drosophila developmental gene. Southern blots containing Bg/II-restricted genomic DNA, which exhibited an allelic restriction fragment length polymorphism for nm23-H1, contained nonallelic bands upon rehybridization to the nm23-H2 probe. Thus, nm23-H1 and nm23-H2 are distinct genes. Northern blot hybridization of nm23-H1- and nm23-H2-specific probes to breast tumors and cell lines indicated that nm23-H1 expression was reduced in high metastatic potential tumor cells to a greater extent than nm23-H2. The data indicate that existence of a family independently regulated nm23 genes. |
| Document Type: Note |
| Language: English |
Addresses:
1. NCI, PATHOL LAB, BETHESDA, MD 20892 USA
2. MOLEC ONCOL INC, GAITHERSBURG, MD 20878 USA |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: EQ683 |
| ISSN: 0008-5472 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |