| | |  | | | | Record from Web of Science® | | | | | | |
| PARTICIPATION OF THE TATA FACTOR IN TRANSCRIPTION OF THE YEAST U6 GENE BY RNA POLYMERASE-C |
|
|
| Author(s): MARGOTTIN F, DUJARDIN G, GERARD M, EGLY JM, HUET J, SENTENAC A |
| Source: SCIENCE Volume: 251 Issue: 4992 Pages: 424-426 Published: JAN 25 1991 |
| Times Cited: 148 References: 33 |
| Abstract: Fractionation of transcription extracts has led to the identification of multiple transcription factors specific for each form of nuclear RNA polymerase. Accurate transcription in vitro of the yeast U6 RNA gene by RNA polymerase C requires at least two factors. One of them was physically and functionally indistinguishable from transcription factor IID (TFIID or BTFI), a pivotal component of polymerase B transcription complexes, which binds to the TATA element. Purified yeast TFIID (yIID) or bacterial extracts that contained recombinant yIID were equally competent to direct specific transcription of the U6 gene by RNA polymerase C. The results suggest the formation of a hybrid transcription machinery, which may imply an evolutionary relation between class B and class C transcription factors. |
| Document Type: Article |
| Language: English |
| Reprint Address: MARGOTTIN, F (reprint author), CENS, DEPT BIOL, SERV BIOCHIM, F-91191 GIF SUR YVETTE, FRANCE |
Addresses:
1. CNRS, GENET MOLEC EUCARYOTES LAB, INSERM, U184, F-67085 STRASBOURG, FRANCE |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: EU504 |
| ISSN: 0036-8075 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |