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| IMPAIRMENT OF GLYCEROL PHOSPHATE SHUTTLE IN ISLETS FROM RATS WITH DIABETES INDUCED BY NEONATAL STREPTOZOCIN |
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| Author(s): GIROIX MH, RASSCHAERT J, BAILBE D, LECLERCQMEYER V, SENER A, PORTHA B, MALAISSE WJ |
| Source: DIABETES Volume: 40 Issue: 2 Pages: 227-232 Published: FEB 1991 |
| Times Cited: 83 References: 30 |
| Abstract: In islets from adult rats injected with streptozocin during the neonatal period, the oxidative and secretory responses to D-glucose are more severely affected than those evoked by L-leucine. A possible explanation for such a preferential defect was sought by comparing the rate of aerobic glycolysis, taken as the sum of D-[3,4-C-14]glucose conversion to labeled CO2, pyruvate, and amino acid, with the total glycolytic flux, as judged from the conversion of D-[5-H-3]glucose to (H2O)-H-3. A preferential impairment of aerobic relative to total glycolysis was found in islets from diabetic rats incubated at either low or high D-glucose concentration. This coincided in islet mitochondria of diabetic rats with a severe decrease in both the basal (no-Ca2+) generation of (H2O)-H-3 from L-[2-H-3]glycerol-3-phosphate and the Ca2+-induced increment in [H-3]glycerophosphate detritiation. The mitochondria of diabetic rats were also less efficient than those of control animals in generating (CO2)-C-14 from [1-C-14]-2-ketoglutarate. The diabetes-induced alteration of 2-ketogluterate dehydrogenase in islet mitochondria was less marked, however, than that of the FAD-linked glycerophosphate dehydrogenase and was not associated with any change in responsiveness to Ca2+. Sonicated islet mitochondria of diabetic rats displayed normal to slightly elevated glutamate dehydrogenase activity. We propose, therefore, that the preferential impairment of the oxidative and secretory responses of islet cells to D-glucose in this experimental model of diabetes may be at least partly attributable to an altered transfer of reducing equivalents into the mitochondria as mediated by the glycerol phosphate shuttle. |
| Document Type: Article |
| Language: English |
Addresses:
1. FREE UNIV BRUSSELS, EXPTL MED LAB, BLVD WATERLOO 115, B-1000 BRUSSELS, BELGIUM
2. UNIV PARIS 07, DEV PHYSIOL LAB, F-75221 PARIS 05, FRANCE |
| Publisher: AMER DIABETES ASSOC, 1660 DUKE ST, ALEXANDRIA, VA 22314 |
| Subject Category: Endocrinology & Metabolism |
| IDS Number: EU663 |
| ISSN: 0012-1797 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |