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| EFFECTS OF INVITRO UVA IRRADIATION AND PUVA TREATMENT ON MEMBRANE FATTY-ACIDS AND ACTIVITIES OF ANTIOXIDANT ENZYMES IN HUMAN KERATINOCYTES |
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| Author(s): PUNNONEN K, JANSEN CT, PUNTALA A, AHOTUPA M |
| Source: JOURNAL OF INVESTIGATIVE DERMATOLOGY Volume: 96 Issue: 2 Pages: 255-259 Published: FEB 1991 |
| Times Cited: 90 References: 36 |
| Abstract: Human Keratinocytes (NCTC 2544) in culture were exposed to either plain ultraviolet A (UVA) irradiation or to 8-methoxypsoralen plus UVA (PUVA) treatment. Lipid peroxidation, activities of antioxidant enzymes, and percentage amounts of C-14-arachidonic acid in various cellular lipid subclasses and in the culture medium were measured. Both UVA irradiation and PUVA treatment induced significant changes in the distribution of arachidonic acid and increased the liberation of arachidonic acid from membrane phospholipids. At 24 h after either UVA irradiation or PUVA treatment the formation of thiobarbituric acid reactive material was significantly increased, whereas the amount of conjugated dienes was unaffected. The activities of he antioxidant enzymes, catalase superoxide dismutase, were already significantly decreased at 0.5 h after UVA irradiation or PUVA treatment. The enzyme activities were partially restored during the following 24 h incubation. From the present study, we suggest that in keratinocytes both plain UVA irradiation and PUVA treatment induce changes in the distribution of membrane fatty acids and cause an impairment in the enzymatic defense system against oxidative stress. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV TURKU, DEPT DERMATOL, SF-20500 TURKU 50, FINLAND
2. UNIV TURKU, DEPT PHYSIOL, SF-20500 TURKU 50, FINLAND |
| Publisher: BLACKWELL SCIENCE INC, 238 MAIN ST, CAMBRIDGE, MA 02142 |
| Subject Category: Dermatology |
| IDS Number: EV774 |
| ISSN: 0022-202X |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |