| EXPRESSION OF HEPATITIS-B VIRUS SURFACE-ANTIGEN IN ADULT-RAT LIVER - CO-INTRODUCTION OF DNA AND NUCLEAR-PROTEIN BY A SIMPLIFIED LIPOSOME METHOD |
|
|
| Author(s): KATO K, NAKANISHI M, KANEDA Y, UCHIDA T, OKADA Y |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 266 Issue: 6 Pages: 3361-3364 Published: FEB 25 1991 |
| Times Cited: 134 References: 25 |
| Abstract: We established a simple and efficient method for gene transfer in vitro (to cultured cells) and in vivo (to an adult organ) using liposomes. Plasmid DNA and proteins were efficiently co-encapsulated in liposomes by agitation and sonication, and were co-introduced into cells by hemagglutinating virus of Japan (HVJ)-mediated membrane fusion. Introduction of the Escherichia coli beta-galactosidase gene with non-histone chromosomal protein high mobility group 1 (HMG1) into LLCMK2 cells resulted in about 3 times higher beta-galactosidase activity than that on introduction of the gene alone. Two days after injection of HVJ-liposomes containing the beta-galactosidase gene and HMG1 under the perisplanchnic membrane of adult rat liver, hepatic cells near the injection site were found by 5-bromo-4-chloro-3-indolyl beta-D-galactoside staining to have beta-galactosidase activity. After similar injection of HVJ-liposomes containing the hepatitis B virus surface antigen (HBsAg) gene and HMG1, HBsAg was detected in the serum for 9 days with a maximum of 25-45 ng/ml on day 2 after the injection. |
| Document Type: Note |
| Language: English |
Addresses:
1. OSAKA UNIV, INST MOLEC & CELLULAR BIOL, 1-3 YAMADAOKA, SUITA, OSAKA 565 JAPAN |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: EY682 |
| ISSN: 0021-9258 |