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| GENOMIC ORGANIZATION OF LIGNIN PEROXIDASE GENES OF PHANEROCHAETE-CHRYSOSPORIUM |
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| Author(s): GASKELL J, DIEPERINK E, CULLEN D |
| Source: NUCLEIC ACIDS RESEARCH Volume: 19 Issue: 3 Pages: 599-603 Published: FEB 11 1991 |
| Times Cited: 35 References: 37 |
| Abstract: Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresis (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome. |
| Document Type: Article |
| Language: English |
Addresses:
1. INST MICROBIAL & BIOCHEM TECHNOL, FOREST PROD LAB, 1 GIFFORD PINCHOT DR, MADISON, WI 53705 USA |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: EY712 |
| ISSN: 0305-1048 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |