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| EXPRESSION OF THE CYSTIC-FIBROSIS GENE IN NONEPITHELIAL INVERTEBRATE CELLS PRODUCES A REGULATED ANION CONDUCTANCE |
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| Author(s): KARTNER N, HANRAHAN JW, JENSEN TJ, NAISMITH AL, SUN SZ, ACKERLEY CA, REYES EF, TSUI LC, ROMMENS JM, BEAR CE, RIORDAN JR |
| Source: CELL Volume: 64 Issue: 4 Pages: 681-691 Published: FEB 22 1991 |
| Times Cited: 475 References: 40 |
| Abstract: The nature of involvement of the cystic fibrosis gene product (CFTR) in epithelial anion transport is not yet understood. We have expressed CFTR in Sf9 insect cells using the baculovirus expression vector system. Reactivity with antibodies against 12 different epitopes spanning the entire sequence suggested that the complete polypeptide chain was synthesized. Immunogold labeling showed localization to both cell-surface and intracellular membranes. Concomitant with CFTR expression, these cells exhibited a new cAMP-stimulated anion permeability. This conductance, monitored both by radioiodide efflux and patch clamping, strongly resembled that present in several CFTR-expressing human epithelial cells. These findings demonstrate that CFTR can function in heterologous nonepithelial cells and lend support to the possibility that CFTR may itself be a regulated anion channel. |
| Document Type: Article |
| Language: English |
| Reprint Address: KARTNER, N (reprint author), HOSP SICK CHILDREN, RES INST, TORONTO M5G 1X8, ONTARIO CANADA |
Addresses:
1. UNIV TORONTO, DEPT PHYSIOL, TORONTO M5S 1A8, ONTARIO CANADA
2. UNIV TORONTO, DEPT MOLEC & MED GENET, TORONTO M5S 1A8, ONTARIO CANADA
3. UNIV TORONTO, DEPT BIOCHEM & CLIN BIOCHEM, TORONTO M5S 1A8, ONTARIO CANADA
4. MCGILL UNIV, DEPT PHYSIOL, MONTREAL H3G 1Y6, QUEBEC CANADA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: EZ478 |
| ISSN: 0092-8674 |
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