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| PHEROMONE-INDUCED PHOSPHORYLATION OF A G-PROTEIN BETA-SUBUNIT IN SACCHAROMYCES-CEREVISIAE IS ASSOCIATED WITH AN ADAPTIVE RESPONSE TO MATING PHEROMONE |
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| Author(s): COLE GM, REED SI |
| Source: CELL Volume: 64 Issue: 4 Pages: 703-716 Published: FEB 22 1991 |
| Times Cited: 82 References: 69 |
| Abstract: The mating pheromone response in S. cerevisiae is activated by a G protein-mediated signaling pathway in which G-beta-gamma is the active transducer of the signal. When exogenous pheromone is added to vegetatively growing cells, G-beta is rapidly phosphorylated at several sites; phosphorylation does not require de novo protein synthesis. A mutation in G-beta was constructed that eliminates signal-induced phosphorylation. This mutation leads to enhanced sensitivity to and impaired ability to recover from pheromone, but does not affect the ability of G-beta-gamma to transmit the mating signal. These phenotypes suggest that G protein phosphorylation mediates an adaptive response to pheromone-induced signaling. G-beta phosphorylation does not require either the pheromone receptor C-terminus or the product of the SST2 gene, both of which mediate separate adaptive responses to pheromone. However, G-beta phosphorylation is greatly facilitated by the presence of the G-alpha subunit, which has also been shown to participate in an adaptation to pheromone. |
| Document Type: Article |
| Language: English |
| Reprint Address: COLE, GM (reprint author), Scripps Res Inst, RES INST, DEPT MOLEC BIOL, MB-7, LA JOLLA, CA 92037 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: EZ478 |
| ISSN: 0092-8674 |
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