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| EXPRESSION OF SMOOTH-MUSCLE CELL PHENOTYPE BY RAT MESANGIAL CELLS IN IMMUNE-COMPLEX NEPHRITIS - ALPHA-SMOOTH MUSCLE ACTIN IS A MARKER OF MESANGIAL CELL-PROLIFERATION |
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| Author(s): JOHNSON RJ, IIDA H, ALPERS CE, MAJESKY MW, SCHWARTZ SM, PRITZL P, GORDON K, GOWN AM |
| Source: JOURNAL OF CLINICAL INVESTIGATION Volume: 87 Issue: 3 Pages: 847-858 Published: MAR 1991 |
| Times Cited: 399 References: 62 |
| Abstract: Mesangial cell proliferation is common in glomerulonephritis but it is unclear if proliferation is associated with any in vivo alteration in phenotype. We investigated whether mesangial cells acquire smooth muscle-like characteristics in a rat model of mesangial proliferative nephritis induced with antibody to the Thy-1 antigen present on mesangial cells. At day 3 glomeruli displayed de novo immunostaining for alpha-smooth muscle actin in a mesangial pattern, correlating with the onset of proliferation, and persisting until day 14. An increase in desmin and vimentin in mesangial regions was also noted. Immunoelectron microscopy confirmed that the actin-positive cells were mesangial cells, and double immunolabeling demonstrated that the smooth muscle actin-positive cells were actively proliferating. Northern analysis of isolated glomerular RNA confirmed an increase in alpha and beta/gamma-actin mRNA at days 3 and 5. Complement depletion or platelet depletion prevented or reduced proliferation, respectively; these maneuvers also prevented smooth muscle actin and actin gene expression. Studies of five other experimental models of nephritis confirmed that smooth muscle actin expression is a marker for mesangial cell injury. Thus, mesangial cell proliferation in glomerulonephritis in the rat is associated with a distinct phenotypic change in which mesangial cells assume smooth muscle cell characteristics. |
| Document Type: Article |
| Language: English |
| Reprint Address: JOHNSON, RJ (reprint author), UNIV WASHINGTON, DEPT MED, DIV NEPHROL, MAIL STOP RM-11, SEATTLE, WA 98195 USA |
Addresses:
1. UNIV WASHINGTON, DEPT PATHOL, SEATTLE, WA 98195 USA |
| Publisher: ROCKEFELLER UNIV PRESS, 222 E 70TH STREET, NEW YORK, NY 10021 |
| Subject Category: Medicine, Research & Experimental |
| IDS Number: FB171 |
| ISSN: 0021-9738 |
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