| | |  | | | | Record from Web of Science® | | | | | | |
| SPECIFIC-INHIBITION OF K-RAS EXPRESSION AND TUMORIGENICITY OF LUNG-CANCER CELLS BY ANTISENSE RNA |
|
|
| Author(s): MUKHOPADHYAY T, TAINSKY M, CAVENDER AC, ROTH JA |
| Source: CANCER RESEARCH Volume: 51 Issue: 6 Pages: 1744-1748 Published: MAR 15 1991 |
| Times Cited: 164 References: 20 |
| Abstract: A human lung cancer cell line (H460a) with a homozygous spontaneous K-ras mutation was transfected with a recombinant plasmid that synthesizes a 2-kilobase genomic segment of the K-ras protooncogene in antisense orientation. Translation of the mutated K-ras mRNA in H460a cells was specifically inhibited, whereas expression of H-ras and N-ras was unchanged. A 3-fold growth inhibition occurred in H460a cells when expression of the mutated ras p21 protein was down-regulated by antisense RNA. However, cells remained viable despite the absence of K-ras expression. The growth of H460a tumors in nu/nu mice was substantially reduced by expressed K-ras antisense RNA. |
| Document Type: Note |
| Language: English |
Addresses:
1. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT THORAC SURG, 1515 HOLCOMBE BLVD, BOX 109, HOUSTON, TX 77030 USA
2. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT TUMOR BIOL, HOUSTON, TX 77030 USA |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: FB576 |
| ISSN: 0008-5472 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |