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| QUANTITATIVE ELECTRONIC IMAGING OF GEL FLUORESCENCE WITH CCD CAMERAS - APPLICATIONS IN MOLECULAR-BIOLOGY |
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| Author(s): SUTHERLAND JC, SUTHERLAND BM, EMRICK A, MONTELEONE DC, RIBEIRO EA, TRUNK J, SON M, SERWER P, PODDAR SK, MANILOFF J |
| Source: BIOTECHNIQUES Volume: 10 Issue: 4 Pages: 492-496 Published: APR 1991 |
| Times Cited: 39 References: 32 |
| Abstract: Electronic imaging systems using charge-coupled-device detectors can replace photographic detection of ethidium bromide-labeled DNA and other fluorophore-labeled biopolymers in electrophoretic gels. The linear response of these devices to fluorescence greatly facilitates quantitative analysis of electrophoretic patterns. Electronic imaging also eliminates several slow and labor-intensive steps inherent in photographic detection of gel fluorescence and simplifies archival storage and recall of gel images. We describe several applications of electronic imaging of gel fluorescence in molecular biology. |
| Document Type: Article |
| Language: English |
| Reprint Address: SUTHERLAND, JC (reprint author), BROOKHAVEN NATL LAB, DEPT BIOL, UPTON, NY 11973 USA |
Addresses:
1. UNIV TEXAS, HLTH SCI CTR, SAN ANTONIO, TX 78284 USA
2. UNIV ROCHESTER, SCH MED, ROCHESTER, NY 14627 USA |
| Publisher: EATON PUBLISHING CO, 154 E. CENTRAL ST, NATICK, MA 01760 |
| Subject Category: Biochemical Research Methods; Biochemistry & Molecular Biology |
| IDS Number: FG600 |
| ISSN: 0736-6205 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |