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| SYNTHESIS FROM DNA OF A MOLECULE WITH THE CONNECTIVITY OF A CUBE |
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| Author(s): CHEN JH, SEEMAN NC |
| Source: NATURE Volume: 350 Issue: 6319 Pages: 631-633 Published: APR 18 1991 |
| Times Cited: 337 References: 15 |
| Abstract: A PRINCIPAL goal of biotechnology is the assembly of novel biomaterials for analytical, industrial and therapeutic purposes. The advent of stable immobile nucleic acid branched junctions 1-4 makes DNA a good candidate for building frameworks to which proteins or other functional molecules can be attached and thereby juxtaposed 5-7. The addition of single-stranded 'sticky' ends 8 to branched DNA molecules converts them into macromolecular valence clusters that can be ligated together 1. The edges of these frameworks are double-helical DNA, and the vertices correspond to the branch points of junctions. Here, we report the construction from DNA of a covalently closed cube-like molecular complex containing twelve equal-length double-helical edges arranged about eight vertices. Each of the six 'faces' of the object is a single-stranded cyclic molecule, doubly catenated to four neighbouring strands, and each vertex is connected by an edge to three others. Each edge contains a unique restriction site for analytical purposes. This is the first construction of a closed polyhedral object from DNA. |
| Document Type: Article |
| Language: English |
| Reprint Address: CHEN, JH (reprint author), NYU, DEPT CHEM, NEW YORK, NY 10003 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: FH112 |
| ISSN: 0028-0836 |
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