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| TRANSCRIPTION INITIATED BY RNA POLYMERASE-II AND TRANSCRIPTION FACTORS FROM LIVER - STRUCTURE AND ACTION OF TRANSCRIPTION FACTOR-EPSILON AND FACTOR-TAU |
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| Author(s): CONAWAY JW, HANLEY JP, GARRETT KP, CONAWAY RC |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 266 Issue: 12 Pages: 7804-7811 Published: APR 25 1991 |
| Times Cited: 69 References: 56 |
| Abstract: We have fractionated rat liver and identified a set of transcription factors that are essential for accurate initiation by RNA polymerase II. These factors were resolved into five distinct enzyme fractions designated alpha, beta-gamma, delta, epsilon, and tau. Four of these fractions can now be replaced with purified proteins. Alpha and beta-gamma were previously purified to apparent homogeneity (Conaway, J. W., and Conaway, R. C. (1989) J. Biol. Chem. 264, 2357-2362). Here, we report purification to near homogeneity of transcription factor-epsilon. Epsilon has a native molecular mass of approximately 90 kDa and is composed of 34- and 58-kDa polypeptides. Both the 34- and 58-kDa polypeptides are required for runoff transcription. In addition, we show that transcription factor-tau is a rat liver homologue of the TATA factor (TFIID or BTF1) that can be efficiently replaced in transcription in vitro by recombinant yeast TFIID. Comparison of the two factors reveals, however, that they differ significantly in their abilities to direct the transcription system to discriminate between promoters of different sequences. |
| Document Type: Article |
| Language: English |
| Reprint Address: CONAWAY, JW (reprint author), OKLAHOMA MED RES FDN, PROGRAM MOLEC & CELL BIOL, OKLAHOMA CITY, OK 73104 USA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: FJ342 |
| ISSN: 0021-9258 |
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