| | |  | | | | Record from Web of Science® | | | | | | |
| IDENTIFICATION OF P53 GENE-MUTATIONS IN BLADDER CANCERS AND URINE SAMPLES |
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| Author(s): SIDRANSKY D, VONESCHENBACH A, TSAI YC, JONES P, SUMMERHAYES I, MARSHALL F, PAUL M, GREEN P, HAMILTON SR, FROST P, VOGELSTEIN B |
| Source: SCIENCE Volume: 252 Issue: 5006 Pages: 706-709 Published: MAY 3 1991 |
| Times Cited: 728 References: 54 |
| Abstract: Although bladder cancers are very common, little is known about their molecular pathogenesis. In this study, invasive bladder cancers were evaluated for the presence of gene mutations in the p53 suppressor gene. Of 18 tumors evaluated, 11 (61 percent) were found to have genetic alterations of p53. The alterations included ten point mutations resulting in single amino acid substitutions, and one 24-base pair deletion. In all but one case, the mutations were associated with chromosome 17p allelic deletions, leaving the cells with only mutant forms of the p53 gene product. Through the use of the polymerase chain reaction and oligomer-specific hybridization, p53 mutations were identified in 1 to 7 percent of the cells within the urine sediment of each of three patients tested. The p53 mutations are the first genetic alterations demonstrated to occur in a high proportion of primary invasive bladder cancers. Detection of such mutations ex vivo has clinical implications for monitoring individuals whose tumor cells are shed extracorporeally. |
| Document Type: Article |
| Language: English |
Addresses:
1. JOHNS HOPKINS UNIV, DEPT ONCOL, BALTIMORE, MD 21231 USA
2. MD ANDERSON HOSP & TUMOR INST, DEPT UROL, HOUSTON, TX 77054 USA
3. UNIV SO CALIF, KENNETH J NORRIS COMPREHENS CANC CTR, LOS ANGELES, CA 90033 USA
4. HARVARD UNIV, SCH MED, DEPT SURG, BOSTON, MA 02115 USA
5. JOHNS HOPKINS UNIV, DEPT UROL, BALTIMORE, MD 21215 USA
6. JOHNS HOPKINS UNIV, DEPT PATHOL, BALTIMORE, MD 21231 USA
7. MD ANDERSON HOSP & TUMOR INST, DEPT CELL BIOL, HOUSTON, TX 77054 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: FK185 |
| ISSN: 0036-8075 |
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