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| RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR TOTAL HOMOCYSTEINE LEVELS IN HUMAN SERUM |
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| Author(s): UBBINK JB, VERMAAK WJH, BISSBORT S |
| Source: JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS Volume: 565 Issue: 1-2 Pages: 441-446 Published: APR 19 1991 |
| Times Cited: 522 References: 24 |
| Abstract: A rapid, isocratic high-performance liquid chromatographic (HPLC) method is described for the determination of total homocysteine levels in human serum. Prior to reversed-phase HPLC analysis, the serum thiols were derivatized with SBD-F (ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate), a thiol-specific fluorogenic probe which is commercially available. Retention of SBD-homocysteine was sensitive to pH, and a mobile phase pH of 2.1 ensured baseline separation of serum thiols within 6 min. The method is simple, sensitive, reproducible (between-run coefficient of variation of 6.6%) and very suitable for routine determination of serum homocysteine levels in a clinical pathology laboratory. |
| Document Type: Note |
| Language: English |
| Reprint Address: UBBINK, JB (reprint author), UNIV PRETORIA, FAC MED, DEPT CHEM PATHOL, POB 2034, PRETORIA 0001, SOUTH AFRICA |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Chemistry, Analytical |
| IDS Number: FK218 |
| ISSN: 0378-4347 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |