| | |  | | | | Record from Web of Science® | | | | | | |
| INTEGRIN-ALPHA-V-BETA-3 AND INTEGRIN-ALPHA-V-BETA-5 CONTRIBUTE TO CELL ATTACHMENT TO VITRONECTIN BUT DIFFERENTIALLY DISTRIBUTE ON THE CELL-SURFACE |
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| Author(s): WAYNER EA, ORLANDO RA, CHERESH DA |
| Source: JOURNAL OF CELL BIOLOGY Volume: 113 Issue: 4 Pages: 919-929 Published: MAY 1991 |
| Times Cited: 299 References: 44 |
| Abstract: We investigated the role of the integrins alpha-v-beta-3 and alpha-v-beta-5 in mediating vitronectin adhesion of three phenotypically distinct cell types. M21 human melanoma cells and H2981 lung carcinoma cells use both alpha-v-containing integrins in adhering to vitronectin while UCLA-P3 lung carcinoma cells adhere exclusively with alpha-v-beta-5. Specifically, monoclonal antibodies directed to functional epitopes on both receptors were required to block adhesion of M21 or H2981 cells while adhesion of UCLA-P3 cells to vitronectin could be blocked with a monoclonal antibody to alpha-v-beta-5. Although both receptors are involved in M21 and H2981 cell adhesion to vitronectin, only alpha-v-beta-3 can be detected in focal contacts, colocalizing with vinculin, talin, and the ends of actin filaments, while alpha-v-beta-5 shows a distinct, nonfocal contact, distribution on the cell surface. These results provide the first evidence that two homologous integrins that recognize the same ligand distribute differentially on the cell surface. |
| Document Type: Article |
| Language: English |
| Reprint Address: WAYNER, EA (reprint author), FRED HUTCHINSON CANC RES CTR, PROGRAM GROWTH REGULAT, 1124 COLUMBIA ST, SEATTLE, WA 98104 USA |
Addresses:
1. ONCOGEN, SEATTLE, WA 98121 USA
2. Scripps Res Inst, RES INST, DEPT IMMUNOL, LA JOLLA, CA 92037 USA |
| Publisher: ROCKEFELLER UNIV PRESS, 222 E 70TH STREET, NEW YORK, NY 10021 |
| Subject Category: Cell Biology |
| IDS Number: FL988 |
| ISSN: 0021-9525 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |