| | |  | | | | Record from Web of Science® | | | | | | |
| EPILIGRIN, A NEW CELL-ADHESION LIGAND FOR INTEGRIN ALPHA-3-BETA-1 IN EPITHELIAL BASEMENT-MEMBRANES |
|
|
| Author(s): CARTER WG, RYAN MC, GAHR PJ |
| Source: CELL Volume: 65 Issue: 4 Pages: 599-610 Published: MAY 17 1991 |
| Times Cited: 500 References: 60 |
| Abstract: Epiligrin is a new glycoprotein in most epithelial basement membranes (BMs) and is a ligand for cell adhesion via integrin alpha-3-beta-1. In the extracellular matrix of human foreskin keratinocytes (HFKs), epiligrin contains three disulfide-bonded, glycoprotein subunits, E170, E145, and E135, based on molecular size in kilo-daltons. Epiligrin, immunopurified with MAb P1E1, induced cell adhesion and localization of integrin alpha-3-beta-1 in focal adhesions (FAs). Cell adhesion to epiligrin was inhibited with an anti-alpha-3-beta-1 MAb. Epiligrin also colocalized with integrin alpha-6-beta-4 in hemidesmosome-like stable anchoring contacts (SACs). Alpha-3-beta-1-FAs encircled alpha-6-beta-4-SACs in a complex adhesion structure. Alpha-3-beta-1 and epiligrin localized in BM junctions of epithelial cells primarily in organs of endodermal/ectodermal origin. In epidermis, epiligrin was detected in the lamina lucida of BMs. Alpha-3-beta-1 localized in plasma membranes of basal cells in contact with epiligrin and also in lateral/apical membranes. Epiligrin is the ligand of an adhesion super complex composed of alpha-3-beta-1-FAs and alpha-6-beta-4-SACs (hemidesmosomes). |
| Document Type: Article |
| Language: English |
| Reprint Address: CARTER, WG (reprint author), UNIV WASHINGTON, FRED HUTCHINSON CANC RES CTR, SEATTLE, WA 98104 USA |
Addresses:
1. UNIV WASHINGTON, DEPT PATHOBIOL, SEATTLE, WA 98104 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: FM037 |
| ISSN: 0092-8674 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |