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| NUCLEOSIDE DIPHOSPHATE KINASE FROM HUMAN ERYTHROCYTES - STRUCTURAL CHARACTERIZATION OF THE 2 POLYPEPTIDE-CHAINS RESPONSIBLE FOR HETEROGENEITY OF THE HEXAMERIC ENZYME |
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| Author(s): GILLES AM, PRESECAN E, VONICA A, LASCU I |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 266 Issue: 14 Pages: 8784-8789 Published: MAY 15 1991 |
| Times Cited: 387 References: 35 |
| Abstract: Human erythrocyte nucleoside-diphosphate kinase (NDP kinase) is a hexameric enzyme consisting of two kinds of polypeptide chains, A and B. By random association (A6, A5B...AB5, B6) these polypeptides form isoenzymes differing in their isoelectric point. Chains A and B of NDP kinase were purified by ion-exchange chromatography under denaturing conditions. Upon mixing and renaturation, the isozymic pattern of NDP kinase obtained by conventional methods was restored. Antibodies raised against purified chains showed significant cross-reactivity, both in immunoblot experiments and activity inhibition studies. Sequence determination showed that both chains consisted of 152 amino acid residues corresponding to M(r) or 17,143 (chain A) and 17,294 (chain B), respectively. There was high homology between the two sequences (88% identity). The phosphorylation site on the enzyme is located at His-118. Chain A was identical with human Nm23 protein, which has been reported as a potential suppressor protein in tumor metastasis and chain B was identical with Nm23-H2 protein. |
| Document Type: Article |
| Language: English |
Addresses:
1. INST PASTEUR, UNITE BIOCHIM REGULAT CELLULAIRES, F-75724 PARIS 15, FRANCE
2. MED & PHARMACEUT INST, DEPT BIOCHEM, R-3400 CLUJ, ROMANIA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: FM038 |
| ISSN: 0021-9258 |
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