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| LEUKOCYTES ROLL ON A SELECTIN AT PHYSIOLOGICAL FLOW-RATES - DISTINCTION FROM AND PREREQUISITE FOR ADHESION THROUGH INTEGRINS |
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| Author(s): LAWRENCE MB, SPRINGER TA |
| Source: CELL Volume: 65 Issue: 5 Pages: 859-873 Published: MAY 31 1991 |
| Times Cited: 1,683 References: 68 |
| Abstract: Rolling of leukocytes on vascular endothelial cells, an early event in inflammation, can be reproduced in vitro on artificial lipid bilayers containing purified CD62, a selectin also named PADGEM and GMP-140 that is inducible on endothelial cells. Neutrophils roll onthis selectin under flow conditions similar to those found in postcapillary venules. Adhesion of resting or activated neutrophils through the integrins LFA-1 and Mac-1 to ICAM-1 in a lipid bilayer does not occur at physiologic shear stresses; however, static incubation of activated neutrophils allows development of adhesion that is greater than 100-fold more shear resistant than found on CD62. Addition of a chemoattractant to activate LFA-1 and Mac-1 results in the arrest of neutrophils rolling on bilayers containing both CD62 and ICAM-1. Thus, at physiologic shear stress, rolling on a selectin is a prerequisite for activation-induced adhesion strengthening through integrins. |
| Document Type: Article |
| Language: English |
| Reprint Address: LAWRENCE, MB (reprint author), HARVARD UNIV, CTR BLOOD RES, 800 HUNTINGTON AVE, BOSTON, MA 02115 USA |
Addresses:
1. HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: FP516 |
| ISSN: 0092-8674 |
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