| | |  | | | | Record from Web of Science® | | | | | | |
| COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT |
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| Author(s): ADAMS MD, KELLEY JM, GOCAYNE JD, DUBNICK M, POLYMEROPOULOS MH, XIAO H, MERRIL CR, WU A, OLDE B, MORENO RF, KERLAVAGE AR, MCCOMBIE WR, VENTER JC |
| Source: SCIENCE Volume: 252 Issue: 5013 Pages: 1651-1656 Published: JUN 21 1991 |
| Times Cited: 1,339 References: 34 |
| Abstract: Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields. |
| Document Type: Article |
| Language: English |
Addresses:
1. NINCDS, RECEPTOR BIOCHEM & MOLEC BIOL SECT, BETHESDA, MD 20892 USA
2. ST ELIZABETH HOSP, NIMH, CTR NEUROSCI, BIOCHEM GENET LAB, WASHINGTON, DC 20032 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: FT114 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |