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| EXPRESSION AND DISTRIBUTION OF RENAL VACUOLAR PROTON-TRANSLOCATING ADENOSINE-TRIPHOSPHATASE IN RESPONSE TO CHRONIC ACID AND ALKALI LOADS IN THE RAT |
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| Author(s): BASTANI B, PURCELL H, HEMKEN P, TRIGG D, GLUCK S |
| Source: JOURNAL OF CLINICAL INVESTIGATION Volume: 88 Issue: 1 Pages: 126-136 Published: JUL 1991 |
| Times Cited: 174 References: 75 |
| Abstract: Renal hydrogen ion excretion increases with chronic acid loads and decreases with alkali loads. We examined the mechanism of adaptation by analyzing vacuolar proton-translocating adenosine triphosphatase (H+ ATPase) 31-kD subunit protein and mRNA levels, and immunocytochemical distribution in kidneys from rats subjected to acid or alkali loads for 1,3,5,7, and 14 d. Acid- and alkali-loaded rats exhibited adaptive responses in acid excretion, but showed no significant changes in H+ ATPase protein or mRNA levels in either cortex or medulla. In contrast, there were profound adaptive changes in the immunocytochemical distribution of H+ ATPase in collecting duct intercalated cells. In the medulla, H+ ATPase staining in acid-loaded rats shifted from cytoplasmic vesicles to plasma membrane, whereas in alkali-loaded rats, cytoplasmic vesicle staining was enhanced, and staining of plasma membrane disappeared. In the cortical collecting tubule, acid loading increased the number of intercalated cells showing enhanced apical H+ ATPase staining and decreased the number of cells with basolateral or poorly polarized apical staining. The results indicate that both medulla and cortex participate in the adaptive response to acid and alkali loading by changing the steady-state distribution of H+ ATPase, employing mechanisms that do not necessitate postulating interconversion of intercalated cells with opposing polarities. |
| Document Type: Article |
| Language: English |
| Reprint Address: BASTANI, B (reprint author), JEWISH HOSP ST LOUIS, DIV RENAL, 216 S KINGSHIGHWAY BLVD, ST LOUIS, MO 63110 USA |
Addresses:
1. WASHINGTON UNIV, SCH MED, DEPT MED, ST LOUIS, MO 63110 USA
2. WASHINGTON UNIV, SCH MED, DEPT CELL BIOL & PHYSIOL, ST LOUIS, MO 63110 USA |
| Publisher: ROCKEFELLER UNIV PRESS, 222 E 70TH STREET, NEW YORK, NY 10021 |
| Subject Category: Medicine, Research & Experimental |
| IDS Number: FU443 |
| ISSN: 0021-9738 |
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