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| CHARACTERIZATION OF THE LEADER PEPTIDE OF AN ENDO-TYPE CELLULASE PRODUCED BY AN ALKALOPHILIC STREPTOMYCES STRAIN |
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| Author(s): PARK JS, HORINOUCHI S, BEPPU T |
| Source: AGRICULTURAL AND BIOLOGICAL CHEMISTRY Volume: 55 Issue: 7 Pages: 1745-1750 Published: JUL 1991 |
| Times Cited: 10 References: 15 |
| Abstract: An endo-type semi-alkaline cellulase (CMCase I) produced by an alkalophilic Streptomyces strain has an extraordinarily long leader peptide of about 70 amino acids (aa), which can be grouped into four distinct regions, an NH2-terminal region (13 aa), an Arg-cluster region (13 aa), a hydrophobic region (23 aa), and an Ala/Pro-repeat region (12 aa). For identification of the function of each part of the leader peptide for secretion of the enzyme, mutations in the leader peptide were generated by site-directed mutagenesis using the cloned gene, and the mutant genes were expressed in 2 cellulase-negative mutant strain, Streptomyces lividans HN1. Although all the alterations in the leader peptide, except for one case, decreased secretion to various extents, in S. lividans, the following conclusions were obtained. Comparison of the intra- and extra-cellular enzyme activities of the mutants suggested that the Arg-cluster region was essential in secretion of the cellulase. Deletion of 8 amino acids rich in threonine and proline in the NH2-terminal region enhanced the secretion to a small extent. Deletion of the Ala/Pro repeat region had almost no effect on secretion. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV TOKYO, DEPT AGR CHEM, BUNKYO KU, TOKYO 113, JAPAN |
| Publisher: JAPAN SOC BIOSCI BIOTECHN AGROCHEM, JAPAN ACAD SOC CTR BLDG, 2-4-6 YAYOI BUNKYO-KU, TOKYO 113, JAPAN |
| Subject Category: Agronomy; Biochemistry & Molecular Biology |
| IDS Number: FZ461 |
| ISSN: 0002-1369 |
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