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| IDENTIFICATION OF FAP LOCUS GENES FROM CHROMOSOME-5Q21 |
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| Author(s): KINZLER KW, NILBERT MC, SU LK, VOGELSTEIN B, BRYAN TM, LEVY DB, SMITH KJ, PREISINGER AC, HEDGE P, MCKECHNIE D, FINNIEAR R, MARKHAM A, GROFFEN J, BOGUSKI MS, ALTSCHUL SF, HORII A, ANDO H, MIYOSHI Y, MIKI Y, NISHISHO I, NAKAMURA Y |
| Source: SCIENCE Volume: 253 Issue: 5020 Pages: 661-665 Published: AUG 9 1991 |
| Times Cited: 1,466 References: 59 |
| Abstract: Recent studies suggest that one or more genes on chromosome 5q2l are important for the development of colorectal cancers, particularly those associated with familial adenomatous polyposis (F"). To facilitate the identification of genes from this locus, a portion of the region that is tightly linked to FAP was cloned. Six contiguous stretches of sequence (contigs) containing approximately 5.5 Mb of DNA were isolated. Subclones from these contigs were used to identify and position six genes, all of which were expressed in normal colonic mucosa. Two of these genes (APC and MCC) are likely to contribute to colorectal tumorigenesis. The MCC gene had previously been identified by virtue of its mutation in human colorectal tumors. The APC gene was identified in a contig initiated from the MCC gene and was found to encode an unusually large protein. These two closely spaced genes encode proteins predicted to contain coiled-coil regions. Both genes were also expressed in a wide variety of tissues. Further studies of MCC and APC and their potential interaction should prove useful for understanding colorectal neoplasia. |
| Document Type: Article |
| Language: English |
Addresses:
1. JOHNS HOPKINS UNIV, SCH MED, MOLEC GENET LAB, BALTIMORE, MD 21231 USA
2. ICI PLC, PHARMACEUT, MACCLESFIELD SK10 4TG, CHESHIRE ENGLAND
3. CHILDRENS HOSP LOS ANGELES, DEPT PATHOL, LOS ANGELES, CA 90027 USA
4. NATL LIB MED, NATL CTR BIOTECHNOL INFORMAT, BETHESDA, MD 20894 USA
5. JAPANESE FDN CANC RES, INST CANC, DEPT BIOCHEM, TOKYO 170, JAPAN |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GA304 |
| ISSN: 0036-8075 |
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