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| REQUIREMENT FOR MAST-CELL GROWTH-FACTOR FOR PRIMORDIAL GERM-CELL SURVIVAL IN CULTURE |
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| Author(s): DOLCI S, WILLIAMS DE, ERNST MK, RESNICK JL, BRANNAN CI, LOCK LF, LYMAN SD, BOSWELL HS, DONOVAN PJ |
| Source: NATURE Volume: 352 Issue: 6338 Pages: 809-811 Published: AUG 29 1991 |
| Times Cited: 348 References: 26 |
| Abstract: MAST-CELL growth factor (MGF) is encoded by the murine steel (Sl) locus and is a ligand for the tyrosine kinase receptor protein encoded by the proto-oncogene c-kit at the murine dominant white spotting (W) locus. Mutations at both these loci affect mast cells, primordial germ cells (PGCs), haemopoietic stem cells and melanocytes. In many Sl and W mutants, the rapid proliferation of PGC that normally occurs between day 7 and 13.5 of embryonic development fails to occur. As c-kit is expressed in PGCs 1,2 while MGF is expressed in the surrounding mesenchyme 2,3, MGF might promote the proliferation of PGCs. Here we report that MGF is essential for PGC survival in culture, but does not stimulate PGC proliferation. Moreover, whereas both the transmembrane and soluble proteolytic cleavage forms of MGF stimulate mast-cell proliferation, soluble MGF has a relatively limited ability to support survival of PGCs in culture, thus explaining the sterility in mice carrying the steel-dickie (Sl(d)) mutation, which encodes only a soluble form of MGF, and providing a functional role for a transmembrane growth factor. |
| Document Type: Article |
| Language: English |
Addresses:
1. NCI, FREDERICK CANC RES & DEV CTR, ABL BASIC RES PROGRAM, MAMMALIAN GENET LAB, FREDERICK, MD 21702 USA
2. NCI, FREDERICK CANC RES & DEV CTR, ABL BASIC RES PROGRAM, MOLEC MECHANISMS CARCINOGENESIS LAB, FREDERICK, MD 21702 USA
3. IMMUNEX CORP, DEPT EXPTL HEMATOL, SEATTLE, WA 98101 USA
4. IMMUNEX CORP, DEPT MOLEC BIOL, SEATTLE, WA 98101 USA
5. INDIANA UNIV, SCH MED, INDIANAPOLIS, IN 46202 USA
6. UNIV ROME TOR VERGATA 2, DIPARTIMENTO SANITA PUBBL & BIOL CELLULARE, I-00173 ROME, ITALY |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GC964 |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |