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| CRYSTAL-STRUCTURE OF A CAP-DNA COMPLEX - THE DNA IS BENT BY 90-DEGREES |
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| Author(s): SCHULTZ SC, SHIELDS GC, STEITZ TA |
| Source: SCIENCE Volume: 253 Issue: 5023 Pages: 1001-1007 Published: AUG 30 1991 |
| Times Cited: 867 References: 91 |
| Abstract: The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90-degrees. This bend results almost entirely from two 40-degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor-DNA complex provides insights into possible mechanisms of transcription activation. |
| Document Type: Article |
| Language: English |
| Reprint Address: SCHULTZ, SC (reprint author), YALE UNIV, DEPT MOLEC BIOPHYS & BIOCHEM, NEW HAVEN, CT 06511 USA |
Addresses:
1. YALE UNIV, DEPT CHEM, NEW HAVEN, CT 06511 USA
2. YALE UNIV, HOWARD HUGHES MED INST, NEW HAVEN, CT 06511 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GC982 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |