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| THE NEUTROPHIL SELECTIN LECAM-1 PRESENTS CARBOHYDRATE LIGANDS TO THE VASCULAR SELECTINS ELAM-1 AND GMP-140 |
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| Author(s): PICKER LJ, WARNOCK RA, BURNS AR, DOERSCHUK CM, BERG EL, BUTCHER EC |
| Source: CELL Volume: 66 Issue: 5 Pages: 921-933 Published: SEP 6 1991 |
| Times Cited: 545 References: 65 |
| Abstract: LECAM-1 (leukocyte-endothelial cell adhesion molecule 1), the lymphocyte lectin ("selectin") homing receptor for peripheral lymph nodes (PLNs), participates in the earliest interactions of polymorphonuclear neutrophilic leukocytes (PMNs) with inflamed venules. Here, we present evidence that LECAM-1 mediates this function through a novel mechanism - presentation of oligosaccharide ligands to the inducible vascular selectins endothelial-leukocyte adhesion molecule (ELAM-1) and granule membrane protein 140 (GMP-140). PMN, but not lymphocyte, LECAM-1 is modified with the vascular selectin ligand sialyl Lewis x (sLex) and specifically binds ELAM-1-transfected cells. Although only a small fraction of total cell surface sLex, LECAM-1-associated sLex appears to play a prominent role in PMN interactions with cell-associated ELAM-1 and GMP-140, as anti-LECAM-1 monoclonal antibodies or selective removal of cell surface LECAM-1 inhibits PMN binding to vascular selectin transfectants by up to 70%. The enhanced function of LECAM-1-associated sLex may reflect the striking concentration, shown here, of LECAM-1 on PMN surface microvilli, the site of initial cellular contact. |
| Document Type: Article |
| Language: English |
| Reprint Address: PICKER, LJ (reprint author), UNIV TEXAS, DEPT PATHOL, MOLEC PATHOL LAB, SW MED CTR, DALLAS, TX 75235 USA |
Addresses:
1. STANFORD UNIV, MED CTR, SCH MED, DEPT PATHOL, STANFORD, CA 94305 USA
2. VET ADM MED CTR, CTR MOLEC BIOL & MED, PALO ALTO, CA 94304 USA
3. UNIV BRITISH COLUMBIA, ST PAULS HOSP, PULM RES LAB, VANCOUVER V6Z 1Y6, BC CANADA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: GE460 |
| ISSN: 0092-8674 |
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