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| THROMBOXANE STIMULATES SYNTHESIS OF EXTRACELLULAR-MATRIX PROTEINS INVITRO |
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| Author(s): BRUGGEMAN LA, HORIGAN EA, HORIKOSHI S, RAY PE, KLOTMAN PE |
| Source: AMERICAN JOURNAL OF PHYSIOLOGY Volume: 261 Issue: 3 Pages: F488-F494 Part: Part 2 Published: SEP 1991 |
| Times Cited: 58 References: 40 |
| Abstract: The vasoconstrictor eicosanoid thromboxane plays an important role in the pathogenesis of several renal diseases. As an autacoid, its local release alters blood flow and induces platelet aggregation. We report a direct stimulatory effect of thromboxane on extracellular matrix protein production and gene expression in vitro. Treatment of two cell types, differentiated mouse teratocarcinoma cells (F9+) and human glomerular mesangial cells, with two different thromboxane analogues resulted in increased production of components of the extracellular matrix including fibronectin and the basement membrane proteins laminin and type IV collagen. These responses to thromboxane were not the result of a mitogenic effect of thromboxane nor the result of an increase in total cellular protein. The increased production of extracellular matrix proteins was, at least in part, due to an increase in the steady-state level of mRNA for these genes. Furthermore, the effect of thromboxane was markedly inhibited by cotreatment with a thromboxane-receptor antagonist. These results suggest a new potential role for thromboxane as a mediator of the sclerotic and fibrotic responses to injury. |
| Document Type: Article |
| Language: English |
Addresses:
1. NIDR, MOLEC MED SECT, DEV BIOL LAB, BLDG 30, RM 433, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA |
| Publisher: AMER PHYSIOLOGICAL SOC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Physiology |
| IDS Number: GE638 |
| ISSN: 0002-9513 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |