| | |  | | | | Record from Web of Science® | | | | | | |
| LOCALIZATION OF TUMOR-NECROSIS-FACTOR-ALPHA IN SYNOVIAL TISSUES AND AT THE CARTILAGE PANNUS JUNCTION IN PATIENTS WITH RHEUMATOID-ARTHRITIS |
|
|
| Author(s): CHU CQ, FIELD M, FELDMANN M, MAINI RN |
| Source: ARTHRITIS AND RHEUMATISM Volume: 34 Issue: 9 Pages: 1125-1132 Published: SEP 1991 |
| Times Cited: 427 References: 39 |
| Abstract: Using immunoaffinity-purified polyclonal antihuman recombinant tumor necrosis factor-alpha (TNF-alpha) F(ab')2 fragments and immunohistochemical techniques, the cells that make TNF-alpha were localized in the inflamed synovial tissue of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Anti-TNF-alpha antibody-stained cells were demonstrated in 9 of 11 RA and 2 of 4 OA but none of 5 normal synovial membranes examined. In RA, 26-64% of the lining layer cells were positive for TNF-alpha. In the interaggregate area, 10-30% of the cells contained TNF-alpha, often in a perivascular distribution, and up to 19% of the cells in lymphoid aggregates stained for TNF-alpha. Some endothelial cells also stained with these antibodies. In OA tissues, the TNF-alpha-containing cells were found predominantly in the deeper layer. Cells containing TNF-alpha were also found at the cartilage-pannus junction in all 4 RA specimens examined. Double immunofluorescence analysis demonstrated that most TNF-alpha-secreting cells in the RA synovial membrane expressed the monocyte/macrophage marker antigens CD11b and CD14, and a few expressed the T cell marker CD3. Our findings provide histologic evidence that TNF-alpha is locally produced in the lining and deeper layers of the synovium by cells of the monocyte/macrophage lineage, supporting its role in inflammation. Further, our findings demonstrate that TNF-alpha is produced by cells at the cartilage-pannus junction, which could affect chondrocyte metabolism, leading to the cartilage degradation in RA. |
| Document Type: Article |
| Language: English |
Addresses:
1. MATHILDA & TERENCE KENNEDY INST RHEUMATOL, DIV CLIN IMMUNOL, 6 BUTE GARDENS, LONDON W6 7DW, ENGLAND
2. CHARING CROSS SUNLEY MED RES CTR, LONDON, ENGLAND |
| Publisher: LIPPINCOTT-RAVEN PUBL, 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 |
| Subject Category: Rheumatology |
| IDS Number: GG337 |
| ISSN: 0004-3591 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |