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| HUMAN CYCLIN-E, A NEW CYCLIN THAT INTERACTS WITH 2 MEMBERS OF THE CDC2 GENE FAMILY |
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| Author(s): KOFF A, CROSS F, FISHER A, SCHUMACHER J, LEGUELLEC K, PHILIPPE M, ROBERTS JM |
| Source: CELL Volume: 66 Issue: 6 Pages: 1217-1228 Published: SEP 20 1991 |
| Times Cited: 543 References: 77 |
| Abstract: A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells. |
| Document Type: Article |
| Language: English |
| Reprint Address: KOFF, A (reprint author), FRED HUTCHINSON CANC RES CTR, DEPT BASIC SCI, 1124 COLUMBIA ST, SEATTLE, WA 98104 USA |
Addresses:
1. ROCKEFELLER UNIV, NEW YORK, NY 10021 USA
2. UNIV RENNES 1, BIOL & GENET DEV LAB, CNRS, UNITE 256, F-35042 RENNES, FRANCE |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: GG552 |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |