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| CONCENTRATION-DEPENDENT TRANSCRIPTIONAL ACTIVATION OR REPRESSION BY KRUPPEL FROM A SINGLE BINDING-SITE |
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| Author(s): SAUER F, JACKLE H |
| Source: NATURE Volume: 353 Issue: 6344 Pages: 563-566 Published: OCT 10 1991 |
| Times Cited: 113 References: 32 |
| Abstract: ONE of the gap class of segmentation genes 1, Kruppel (Kr), is required for normal thorax and abdominal development of the Drosophila embryo. Its gene product, a zinc-finger type protein 2, forms a bell-shape concentration gradient in a central position of the blastoderm 3-6. Genetic and molecular studies suggested that the Kr protein (KR) may act both as a positive and as a negative regulator of transcription on several other genes 3-9 of the zygotic segmentation hierarchy 1. We have examined the regulatory potential of Kr by a series of cotransfection experiments in the Drosophila Schneider cell line system. Different doses of Kr expression plasmid were tested for their ability to drive reporter gene expression mediated by a single 11-base pair KR in vitro binding site common to several putative Kr target genes 4-6,8-10. Our results show that low amounts of Kr expression plasmid lead to transcriptional activation, whereas high amounts result in repression. Distinct portions of KR other than the DNA-binding domain are required for gene activation and repression, suggesting that KR itself can act as a concentration-dependent positive and negative regulator of transcription. |
| Document Type: Article |
| Language: English |
| Reprint Address: SAUER, F (reprint author), MAX PLANCK INST BIOPHYS CHEM, MOLEK ENTWICKLUNGSBIOL ABT, FASSBERG, W-3400 GOTTINGEN, GERMANY |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GJ643 |
| ISSN: 0028-0836 |
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