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| EFFECT OF STEEL FACTOR AND LEUKEMIA INHIBITORY FACTOR ON MURINE PRIMORDIAL GERM-CELLS IN CULTURE |
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| Author(s): MATSUI Y, TOKSOZ D, NISHIKAWA S, NISHIKAWA SI, WILLIAMS D, ZSEBO K, HOGAN BLM |
| Source: NATURE Volume: 353 Issue: 6346 Pages: 750-752 Published: OCT 24 1991 |
| Times Cited: 309 References: 28 |
| Abstract: DESPITE the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis 1-5. Mutations in the murine Dominant White Spotting (W) and Steel genes, which respectively encode the c-kit tyrosine kinase receptor and the c-kit ligand (or Steel factor), impair the development of primordial germ cells (PGCs) in vivo, as well as haematopoietic stem cells and neural crest-derived melanoblast 3,6-16. Here we use a monoclonal antibody against c-kit tyrosine kinase receptor and recombinant Steel factor to study the c-kit receptor-ligand system in cultured PGCs. In addition, we show that leukaemia inhibitory factor (also known as differentiation inhibitory activity) 17,18, a factor secreted by STO fibroblasts, can stimulate proliferation of primordial germ cells in vitro. |
| Document Type: Article |
| Language: English |
Addresses:
1. VANDERBILT UNIV, MED CTR, SCH MED, DEPT CELL BIOL, NASHVILLE, TN 37232 USA
2. HARVARD UNIV, SCH MED, DANA FARBER CANC INST, BOSTON, MA 02115 USA
3. CHILDRENS HOSP MED CTR, HOWARD HUGHES MED INST, BOSTON, MA 02115 USA
4. KUMAMOTO UNIV, INST MED IMMUNOL, DEPT PATHOL, KUMAMOTO 860, JAPAN
5. AMGEN INC, AMGEN CTR, THOUSAND OAKS, CA 91320 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GL696 |
| ISSN: 0028-0836 |
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