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| SKIN WOUND CLOSURE IN ATHYMIC MICE WITH CULTURED HUMAN-CELLS, BIOPOLYMERS, AND GROWTH-FACTORS |
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| Author(s): BOYCE ST, FOREMAN TJ, ENGLISH KB, STAYNER N, COOPER ML, SAKABU S, HANSBROUGH JF |
| Source: SURGERY Volume: 110 Issue: 5 Pages: 866-876 Published: NOV 1991 |
| Times Cited: 53 References: 55 |
| Abstract: Skin wound closure remains a major problem in acute and reconstructive skin grafting after large burns because of limited availability of donor skin. This report evaluates six protocols for preparation in vitro of skin substitutes composed of cultured human cells, biopolymers, and growth factors for wound closure. Full-thickness wounds in athymic mice treated in a single procedure with cultured skin substitutes were compared directly to treatments with murine skin autograft, human skin xenograft, or no graft. Rectilinear planimetry of healed wounds 6 weeks after surgery showed that skin substitutes cultured in serum-free medium, and for 24 hours before surgery in defined medium with basic fibroblast growth factor (100 ng/ml), were not statistically different (p < 0.05) in size from treatment with human skin xenograft. Acceptance and persistence of skin substitutes cultured in serum-free media were 70% at 6 weeks after surgery, as determined by staining of healed skin with a fluorescein-labeled monoclonal antibody against human HLA-ABC antigens. Ultrastructural examination of wounds with cultured human skin 6 weeks after treatment showed complete basement membrane, including anchoring fibrils, presence of melanocytes and pigment transfer to keratinocytes, and innervation of healed skin adjacent to basement membrane. These findings demonstrate effectiveness of cultured skin substitutes for closure of skin wounds and illustrate important capabilities to modulate the natural processes of wound repair, to increase supply of materials used for wound repair, and to enhance quality of wound healing. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV CALIF SAN DIEGO, MED CTR, DEPT SURG, SAN DIEGO, CA 92103 USA
2. UNIV UTAH, DEPT PHYSIOL, SALT LAKE CITY, UT 84112 USA |
| Publisher: MOSBY-YEAR BOOK INC, 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 |
| Subject Category: Surgery |
| IDS Number: GM945 |
| ISSN: 0039-6060 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |