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| DNA-BINDING PROPERTIES OF THE PRODUCT OF THE TESTIS-DETERMINING GENE AND A RELATED PROTEIN |
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| Author(s): NASRIN N, BUGGS C, KONG XF, CARNAZZA J, GOEBL M, ALEXANDERBRIDGES M |
| Source: NATURE Volume: 354 Issue: 6351 Pages: 317-320 Published: NOV 28 1991 |
| Times Cited: 159 References: 17 |
| Abstract: THE upstream region of the human glyceraldehyde-3-phosphate dehydrogenase gene contains an insulin-response element (IRE-A) responsible for insulin-dependent transcription of the gene (ref. 1). The open reading frame of a rat complementary DNA encoding a protein (IRE-ABP) that binds to this sequence contains an HMG box motif 2 that is 67% identical to the mouse candidate gene for the testis-determining factor SRY, and 98% identical to the mouse SRY-like gene, alpha-4(refs 3, 4). Here we report that IRE-ABP and SRY bind to IRE-A DNA with comparable specificity in a DNase-I footprinting assay. Two females with sex reversal were found to have a single amino-acid substitution in the HMG box domain of SRY at position 3 and 7, respectively 5,6. SRY derivatives containing corresponding mutations do not make contact with IRE-A DNA. These results are direct evidence that mouse SRY-like proteins are sequence-specific DNA-binding proteins and identify two amino acids critical to this interaction. Moreover, IRE-A is a candidate SRY-response element. |
| Document Type: Article |
| Language: English |
Addresses:
1. HARVARD UNIV, MASSACHUSETTS GEN HOSP, SCH MED, HOWARD HUGHES MED INST, DIABET UNIT, 50 BLOSSOM ST, BOSTON, MA 02114 USA
2. HARVARD UNIV, MASSACHUSETTS GEN HOSP, SCH MED, MED SERV, BOSTON, MA 02114 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GT204 |
| ISSN: 0028-0836 |
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