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| SEQUENCE-SELECTIVE RECOGNITION OF DNA BY STRAND DISPLACEMENT WITH A THYMINE-SUBSTITUTED POLYAMIDE |
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| Author(s): NIELSEN PE, EGHOLM M, BERG RH, BUCHARDT O |
| Source: SCIENCE Volume: 254 Issue: 5037 Pages: 1497-1500 Published: DEC 6 1991 |
| Times Cited: 1,473 References: 29 |
| Abstract: A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization. |
| Document Type: Article |
| Language: English |
| Reprint Address: NIELSEN, PE (reprint author), PANUM INST, DEPT BIOCHEM B, MED BIOTECHNOL RES CTR, BLEGDAMSVEJ 3C, DK-2200 COPENHAGEN, DENMARK |
Addresses:
1. HC ORSTED INST, DEPT ORGAN CHEM, DK-2100 COPENHAGEN 0, DENMARK
2. RISO NATL LAB, DEPT MAT, POLYMER GRP, DK-4000 ROSKILDE, DENMARK |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GT903 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |